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on the other side of a semipermeable membrane
(the inside of the RBC); the medium would pos-
sess a smaller concentration of particles and con-
sequently would lose water during osmosis. In
our case, the red blood cells were hypertonic in
relation to the medium and exerted a larger
osmotic pull than the medium on the other side of
the semipermeable membrane because it pos-
sessed a greater concentration of particles and
consequently accumulated water during osmosis.
158.
D.
In this experiment, urea and glycerol did
enter the red blood cell and water consequently
followed because it was in greater quantity out-
side and tried to establish an equilibrium.
159.
D.
Cell membranes are described as semiperme-
able because they allow some materials to pass
while they block others. All materials that pass
membranes must be either a liquid or dissolved in
a liquid. Molecules also must be fairly simple, but
molecular size is not the limiting factor because
amino acids pass less readily than do many
smaller molecules. Osmosis is the passage of liq-
uids through a membrane; usually water through
the semipermeable membrane. Diffusion is the
passage of molecules from a more concentrated
environment to a less concentrated region; equal-
ization is usually the end result.
160.
D.
Active transport involves the diffusion of
molecules against a gradient; this is an energy
consuming phenomenon. The process of mov-
ing substances from an area of lower concentra-
tion to where they are in a higher concentration
is selective, and requires respiration. Osmosis
and diffusion are described in the explanation
for question 159. Turgor pressure involves the
passage of water into a cell at a faster rate than
it can leave; the cell becomes plump and filled
(turgid). At times the force results in bursting
the structures. Plasmolysis is defined as a
shrinking of protoplasm due to the loss of water
from a cell.
161.
A.
Layers of material (probably mucopolysac-
charide) secreted by the cell are found on the sur-
face of the cell. The most prominent layer is the
basement membrane, or basal lamina. These struc-
tures are boundaries and must be traversed by mate-
rial entering and leaving the cell. Cells must be held
together; adjacent cell membranes interdigitate and
intercellular cement is utilized. A desmosome is a
specialized area of connection between adjacent
cellular membranes (macula adherens). A terminal
bar is a dense area surrounding the apical cellular
surface. It includes the tight junction (zona occlu-
dens) and the loose junction (zona adherens). In car-
diac muscle, several cardiac muscle cells join end to
end at a specialized junctional zone known as an
intercalated disc.
162.
B.
Some of the proteins embedded in the lipid
layers of membranes are shaped to form channels
with "gates," which open only to certain materials
or under certain conditions.
163.
A.
Osmosis is a process in which solvent passes
from an area of lower solute concentration to an
area of higher solute concentration. In dialysis sol-
vent and solute both pass through the membrane.
164.
A.
The defect cannot be dominant because indi-
vidual 5 was produced by two normal parents who
would have no defective alleles if the defect is
dominant (either autosomal or X-linked). The
defect is unlikely to be X-linked recessive
because unless a new mutation occurred, individ-
ual 5 should have received a normal allele for the
gene on her X from her father (individual 2) and
therefore could not express a recessive X-linked
defect. Therefore the most likely possibility
(using the assumptions of a single controlling
gene and very rare mutations) would be an auto-
somal gene for which the defective allele is reces-
sive to the normal allele.
165.
C.
Unless a new mutation occurred, individual
1 would have to have one normal allele (which
produced the normal phenotype) and one defec-
tive allele (which was contributed to individual 5
along with a defective allele from individual 2) to
produce individual 5's defective phenotype.
166.
B.
Individual 4 would have to contain two defec-
tive alleles to produce the defective phenotype.
167.
C.
Individual 7 would have to contain at least
one normal allele to produce her normal pheno-
type. Her other allele cannot be determined with
certainty from the data given. Because her parents
are probably both heterozygotes (see above), she
has a
1/3 probability of having two normal alleles
and a
2/3 probability of having one normal and
one abnormal allele.
i.e., Nn
Nn
1/4 NN : 2/4 Nn : 1/4 nn
defective phenotype
2/3 of normal phenotype
1/3 of normal phenotype
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