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ocrit, 10 milliliters of blood are collected and placed in
a heparinized centrifuge tube. Heparin is used to pre-
vent clotting. The tube is spun for 15–20 minutes at
2500 rpm to sediment all the formed elements. The
tube is then examined to determine the volume (in mil-
liliters) of the packed formed elements. On the average,
blood consists of approximately 45% formed elements
and approximately 55% plasma; in other words, each
10 ml of blood will contain 4.5 ml of formed elements.
The average "normal" packed cell volume or hemat-
ocrit is therefore 45% (i.e., 4.5 ml is 45% of 10 ml).
Most of the sedimented formed elements are RBCs. A
small region of WBCs and platelets will be located at
the top of the region of formed elements; this layer is
often referred to as the "buffy" coat.
A patient was seen by a physician who ordered a
hematocrit as part of the patient's lab workup. Ten mil-
limeters of blood were collected and treated as previ-
ously described. The diagram below shows the
hematocrit tube at the end of centrifugation.
169.
Region A consists of:
A.
fibrin clot material.
B.
plasma.
C.
serum.
D.
WBCs.
170.
Region B consists predominantly of:
A.
fibrin clot material.
B.
plasma.
C.
RBCs.
D.
WBCs.
171.
The lower 5.5 ml consists predominantly of:
A.
fibrin clot material.
B.
platelets.
C.
RBCs.
D.
WBCs.
10
8
6
4
3
2
1
A
B
C
172.
The hematocrit of this patient is:
A.
45%.
C.
4.5 ml.
B.
55%.
D.
5.5 ml.
173.
The hematocrit of this patient is:
A.
normal.
B.
above normal.
C.
lower than normal.
D.
cannot be determined from the information
given.
174.
The components of the blood responsible for
binding and carrying oxygen would be located
in:
A.
region A.
C.
region C.
B.
region B.
D.
regions A and C.
175.
The cellular components of the blood respon-
sible for fighting infections would be located
in:
A.
region A.
C.
region C.
B.
region B.
D.
regions B and C.
176.
The components of the blood responsible for
initiating blood clotting would be located in:
A.
region A.
C.
region C.
B.
region B.
D.
regions B and C.
177.
The major component found in region A would
be:
A.
water.
C.
sodium chloride.
B.
hemoglobin.
D.
glucose.
178.
The hematocrit of the patient, as compared to the
normal value, suggests that the patient:
A.
lives at a high altitude.
B.
has sickle cell anemia.
C.
has pernicious anemia.
D.
has recently undergone excessive X ray
treatment.
Passage VI (Questions 179–183)
A muscle fiber is a single muscle cell. Each fiber is
composed of numerous cylindrical fibrils running the
entire length of the fiber. The fibril exhibits light and
dark bands--I and A bands respectively. The I band is
bisected by the M line. There is a somewhat lighter
band within the A band that is called the H band. These
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